{"id":10417,"date":"2026-02-16T15:08:55","date_gmt":"2026-02-16T15:08:55","guid":{"rendered":"https:\/\/corbeauinnovation.com\/staging\/3392\/?page_id=10417"},"modified":"2026-02-18T14:44:51","modified_gmt":"2026-02-18T14:44:51","slug":"fluospec","status":"publish","type":"page","link":"https:\/\/corbeauinnovation.com\/staging\/3392\/fluospec\/","title":{"rendered":"Fluospec platereader"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"10417\" class=\"elementor elementor-10417\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8ae369b e-grid e-con-boxed e-con e-parent\" data-id=\"8ae369b\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;sticky&quot;:&quot;top&quot;,&quot;sticky_on&quot;:[&quot;desktop&quot;,&quot;tablet&quot;,&quot;mobile&quot;],&quot;sticky_offset&quot;:0,&quot;sticky_effects_offset&quot;:0,&quot;sticky_anchor_link_offset&quot;:0}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5da9c00 elementor-widget elementor-widget-theme-site-title elementor-widget-heading\" data-id=\"5da9c00\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"theme-site-title.default\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\"><a href=\"https:\/\/corbeauinnovation.com\/staging\/3392\">Corbeau Innovation<\/a><\/h1>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3d3fec6 e-full_width e-n-menu-layout-horizontal e-n-menu-tablet elementor-widget elementor-widget-n-menu\" data-id=\"3d3fec6\" data-element_type=\"widget\" data-e-type=\"widget\" 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id=\"e-n-menu-title-6422\" class=\"e-n-menu-title\">\n\t\t\t\t\t<a class=\"e-n-menu-title-container e-focus e-link\" href=\"http:\/\/corbeauinnovation.com\/contact-us\/\">\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"e-n-menu-title-text\">\n\t\t\t\t\t\t\tcontact\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t<\/div>\n\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b612ba3 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"b612ba3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-fdc0b48 e-flex e-con-boxed e-con e-parent\" data-id=\"fdc0b48\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-fb5ad83 elementor-widget elementor-widget-text-editor\" data-id=\"fb5ad83\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<h1>Fluospec platereader<\/h1>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-397c30f e-flex e-con-boxed e-con e-parent\" data-id=\"397c30f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-643626b e-grid e-con-full e-con e-child\" data-id=\"643626b\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-02c1e7c elementor-widget elementor-widget-text-editor\" data-id=\"02c1e7c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<h3 class=\"western\">Fluospec platereader is part of a drug discovery platform utilising fluorescent dye tags with long fluorescence lifetimes. Assays with fluorescence lifetime discrimination have a number of important advantages:<\/h3><ul><li><p class=\"western\">fast, convenient assay development with functionalised dye reagents<\/p><\/li><li><p class=\"western\">a range of enzyme substrates and ready-to-run assays for kinase and protease profiling<\/p><\/li><li><p class=\"western\">suppression of interference from coloured and fluorescent compounds<\/p><\/li><li>improved rates of false positives and false negatives compared to simple intensity assays<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-53ade01 elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-pagination-position-inside elementor-widget elementor-widget-slides\" data-id=\"53ade01\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;both&quot;,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;autoplay_speed&quot;:5000,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;,&quot;transition_speed&quot;:500}\" data-widget_type=\"slides.default\">\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper swiper\" role=\"region\" aria-roledescription=\"carousel\" aria-label=\"Slides\" dir=\"ltr\" data-animation=\"fadeInUp\">\n\t\t\t\t<div class=\"swiper-wrapper elementor-slides\">\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-repeater-item-701c4a6 swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"Fluospec platereader\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><div class=\"elementor-slide-heading\">Fluospec platereader<\/div><div class=\"elementor-slide-description\">for biochemical assays<\/div><div  class=\"elementor-button elementor-slide-button elementor-size-sm\">Click Here<\/div><\/div><\/div><\/div><div class=\"elementor-repeater-item-d02725a swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"lyn kinase assay\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><div class=\"elementor-slide-heading\">Kinase assays<\/div><div class=\"elementor-slide-description\">[ATP] of choice without affinity particles<\/div><div  class=\"elementor-button elementor-slide-button elementor-size-sm\">Click Here<\/div><\/div><\/div><\/div><div class=\"elementor-repeater-item-be4af28 swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"caspase 3 assay\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><div class=\"elementor-slide-heading\">Caspase 3 assay<\/div><div class=\"elementor-slide-description\">miniaturise to 1536 well plate<\/div><div  class=\"elementor-button elementor-slide-button elementor-size-sm\">Click Here<\/div><\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper-button elementor-swiper-button-prev\" role=\"button\" tabindex=\"0\" aria-label=\"Previous slide\">\n\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-eicon-chevron-left\" viewBox=\"0 0 1000 1000\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M646 125C629 125 613 133 604 142L308 442C296 454 292 471 292 487 292 504 296 521 308 533L604 854C617 867 629 875 646 875 663 875 679 871 692 858 704 846 713 829 713 812 713 796 708 779 692 767L438 487 692 225C700 217 708 204 708 187 708 171 704 154 692 142 675 129 663 125 646 125Z\"><\/path><\/svg>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<div class=\"elementor-swiper-button elementor-swiper-button-next\" role=\"button\" tabindex=\"0\" aria-label=\"Next slide\">\n\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-eicon-chevron-right\" viewBox=\"0 0 1000 1000\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M696 533C708 521 713 504 713 487 713 471 708 454 696 446L400 146C388 133 375 125 354 125 338 125 325 129 313 142 300 154 292 171 292 187 292 204 296 221 308 233L563 492 304 771C292 783 288 800 288 817 288 833 296 850 308 863 321 871 338 875 354 875 371 875 388 867 400 854L696 533Z\"><\/path><\/svg>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"swiper-pagination\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-48b882a e-flex e-con-boxed e-con e-parent\" data-id=\"48b882a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-af0518c elementor-drop-cap-yes elementor-drop-cap-view-default elementor-widget elementor-widget-text-editor\" data-id=\"af0518c\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;drop_cap&quot;:&quot;yes&quot;}\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p><b>VALIDATE reliably with the FLUOSPEC \u00ae FL plate reader<\/b><br \/>Validating &#8216;hits&#8217; from a primary high throughout compound screen is an important step in removing artefacts from datasets. Screening 1 million compounds at a hit rate of ~1% produces 10,000 validation tests before attractive looking leads can be selected. With such a large number of tests, each comprising perhaps 14 individual measurements, reliability and efficiency is crucial. A leading pharmaceutical company tested the reliability of IC50 values obtained with the\u00a0 \u00a0FLUOSPEC \u00ae FL under normal operating procedures in an automation lab. Excellent reliability was demonstrated.<\/p><p>Twelve point IC50 measurements were made for 394 compounds using a PURETIME \u00ae SRC kinase assay (3\u00b5L final volume in 1536 well plate). Control wells were randomly distributed across the plate and each compound concentration was represented by a single well. Reliability was determined by comparing the derived IC50 values from a duplicate 1536 well plate. A correlation plot was used to compare the IC 50 values obtained from\u00a0single well dose response series from the two plates. The FLUOSPEC \u00ae FL produced highly correlated IC50 values over the whole concentration range from 0.1nM to 100\u00b5M, demonstrating reliable hit validation in an automation lab.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-6e2184d e-flex e-con-boxed e-con e-parent\" data-id=\"6e2184d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-53718cf elementor-widget elementor-widget-image\" data-id=\"53718cf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" loading=\"lazy\" width=\"768\" height=\"524\" src=\"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-content\/uploads\/2026\/02\/IC50-correlation-test-768x524.png\" class=\"attachment-medium_large size-medium_large wp-image-10461\" alt=\"IC50 correlation test\" srcset=\"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-content\/uploads\/2026\/02\/IC50-correlation-test-768x524.png 768w, https:\/\/corbeauinnovation.com\/staging\/3392\/wp-content\/uploads\/2026\/02\/IC50-correlation-test-300x205.png 300w, https:\/\/corbeauinnovation.com\/staging\/3392\/wp-content\/uploads\/2026\/02\/IC50-correlation-test.png 864w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0eff17a e-flex e-con-boxed e-con e-parent\" data-id=\"0eff17a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c559787 elementor-widget__width-initial elementor-widget elementor-widget-video\" data-id=\"c559787\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;youtube_url&quot;:&quot;https:\\\/\\\/www.youtube.com\\\/watch?v=X-Eh4uprqSQ&quot;,&quot;video_type&quot;:&quot;youtube&quot;,&quot;controls&quot;:&quot;yes&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-wrapper elementor-open-inline\">\n\t\t\t<div class=\"elementor-video\"><\/div>\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-e891d73 e-flex e-con-boxed e-con e-parent\" data-id=\"e891d73\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3793ba3 elementor-widget elementor-widget-text-editor\" data-id=\"3793ba3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p><em>Tradeshow demonstration of Fluospec integrated with plate robot<\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>about contact Fluospec platereader Fluospec platereader is part of a drug discovery platform utilising fluorescent dye tags with long fluorescence lifetimes. Assays with fluorescence lifetime discrimination have a number of important advantages: fast, convenient assay development with functionalised dye reagents a range of enzyme substrates and ready-to-run assays for kinase and protease profiling suppression of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"nf_dc_page":"","footnotes":""},"class_list":["post-10417","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-json\/wp\/v2\/pages\/10417","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-json\/wp\/v2\/comments?post=10417"}],"version-history":[{"count":31,"href":"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-json\/wp\/v2\/pages\/10417\/revisions"}],"predecessor-version":[{"id":10509,"href":"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-json\/wp\/v2\/pages\/10417\/revisions\/10509"}],"wp:attachment":[{"href":"https:\/\/corbeauinnovation.com\/staging\/3392\/wp-json\/wp\/v2\/media?parent=10417"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}